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Genome-wide epigenome analysis of KSHV in BCBL-1 cells.

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posted on 17.07.2014, 04:03 authored by Jianhong Hu, Yajie Yang, Peter C. Turner, Vaibhav Jain, Lauren M. McIntyre, Rolf Renne

ChIP assays were performed in BCBL-1 cells with control IgG, H3K4me3, H3K27me3, and Pol II antibody. The resulting ChIP DNA was used for library construction followed by Illumina sequencing. Sequencing tags mapped to viral genome were visualized in UCSC Genome Browser. The number of sequence tags is indicated for each track (y-axis), which represents the number of times each region was recovered by the ChIP-seq. The highest coverage for H3K4me3 is 6,660 within the terminal repeat which was not shown in order to visualize the lower peaks.