Genome organization of three RV vector variants.

Genome organization and the close-up between the end of the M gene and BsiW I site. The transcription unit, start and stop/polyadenylation signals, AACA, and poly(A) are respectively shown as black bars and black arrowheads in the schematic diagram and are shaded yellow in the close-up. LS: leader sequence; TS: trailer sequence. A: rHEP5.0-CVSG-mRFP was constructed based on HEP strain by the replacement of the G gene with the G gene derived from the CVS strain (CVS-G). rHEP5.0-CVSG-mRFP has an mRFP gene as a transgene between the N and P genes and has an additional transcription unit between the G and L genes. B: In rHEP5.0-ΔG-mRFP, the G gene and a transcription unit were deleted from rHEP5.0-CVSG-mRFP. C: rHEP5.0-ΔG-mRFP-BPB had two TagBFP genes, which were linked by a P2A sequence (BPB) as one gene in the place of the G gene.