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Genipin exhibits anti-invasive effect in orthotopically HCC-implanted mice. A

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posted on 2013-02-19, 22:32 authored by Ning Wang, Meifen Zhu, Sai-Wah Tsao, Kwan Man, Zhangjin Zhang, Yibin Feng

shows Genipin has no toxic effect on the orthotopically HCC implanted model. MHCC97L cells were subcutaneously injected into the right flank of the nude mice to allow xenografted tumor growth. Once the solid tumor reaches about 1 cm in diameter, it was dissected out and cut into 1–2 mm small cubes, which was then implanted to the left liver lobe of the nude mice to allow orthotopical growth of HCC. Seven days later, the nude mice was randomized into two groups (n = 5). Treatment was conducted seven days after implantation (genipin 30 mg/kg/2 days, i.p.) and lasted for 3 weeks, while control group received same volume of PBS. Body weight of the mice was examined once per two days. No significant loss in body weight during treatment could be observed, indicating genipin may not exhibit potent toxicity to the mice. B shows that genipin treatment could reduce the tumor size in HCC-implanted mice. C shows that genipin suppresses tumor cell invasion to the normal tissue in orthotopically HCC implanted model. The liver of mice was dissected out and histological analysis was conducted with H & E staining. Significantly reduction of intrahepatic invasion of MHCC97L could be observed in genipin-treated mice.

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