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Genetic incorporation of alkene-lysine analogs 1, 2 and 3 into sfGFP.

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posted on 02.09.2014, 03:39 by Jessica Torres-Kolbus, Chungjung Chou, Jihe Liu, Alexander Deiters

(A) SDS-PAGE analysis of purified sfGFP. –AA: no UAA was supplemented; WT: wild-type sfGFP; 1, 2 and 3: expression in the presence of the corresponding UAA (1 mM). (B) Protein yields (*wild-type sfGFP yield is 70 mg/L, 100%) and ESI-MS results.