Figure_4.tif (302.91 kB)
Download file

Genes in the Ski pathway normally suppress FHV replication in yeast.

Download (0 kB)
posted on 21.04.2014, 11:38 authored by Linhui Hao, Brett Lindenbach, Xiaofeng Wang, Billy Dye, David Kushner, Qiuling He, Michael Newton, Paul Ahlquist

Yeast strains with the indicated Ski gene deletions were transformed with plasmid pF1 (Figure 1A) and, following galactose-induction of pF1 transcription, FHV genomic RNA1 replication and subgenomic RNA3 production were assayed by Northern blot hybridization. The histograms show the averages and standard deviations of RNA1 and RNA3 levels across four independent experiments. Representative Northern blots are shown above the histograms.