Genes in the Ski pathway normally suppress FHV replication in yeast.
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Yeast strains with the indicated Ski gene deletions were transformed with plasmid pF1 (Figure 1A) and, following galactose-induction of pF1 transcription, FHV genomic RNA1 replication and subgenomic RNA3 production were assayed by Northern blot hybridization. The histograms show the averages and standard deviations of RNA1 and RNA3 levels across four independent experiments. Representative Northern blots are shown above the histograms.