Generation of increased and decreased HOTAIR expressing gastric carcinoma cells and compared relative expression between cell lines and human gastric tissues.
A, Relative HOTAIR expression levels in gastric cancer cell lines were analyzed by qRT-PCR analysis. MKN-HOTAIR cells expressed about 10 fold higher levels of HOTAIR than parental MKN74 cells and MKN-EV. On the other hand, the expression of HOTAIR in KATO-shHOTAIR cells was reduced by 30 to 70% compared to parental KATO III and KATO-EV cells. B, The bar graph demonstrated the relative expression of HOTAIR in gastric cancer cell lines, normal and carcinoma tissues of the stomach analyzed in this study. Expression of HOTAIR was normalized to that of GAPDH. Values are expressed relative to 1.00 for expression in parental MKN74 cells.