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Generation of Col1a1-Krm2 transgenic mice.

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posted on 21.02.2013, 01:39 by Jochen Schulze, Sebastian Seitz, Hiroaki Saito, Michael Schneebauer, Robert P. Marshall, Anke Baranowsky, Bjoern Busse, Arndt F. Schilling, Felix W. Friedrich, Joachim Albers, Alexander S. Spiro, Jozef Zustin, Thomas Streichert, Kristina Ellwanger, Christof Niehrs, Michael Amling, Roland Baron, Thorsten Schinke

(A) Schematic presentation of the construct used for osteoblast-specific over-expression of Krm2 (top) and identification of three transgenic founder animals (arrows) by Southern Blotting (bottom), one of them dying at the age of 10 weeks. (B) Von Kossa/van Gieson staining of non-decalcified vertebral body sections from 10 weeks old female wildtype mice (n = 3), the dead founder animal (#2) and age-matched female transgenic offspring from the two other founders (n = 3, scale bars, 1 mm). The transgene copy numbers, as well as the trabecular bone volume (BV/TV, bone volume per tissue volume) is given below. (C) Transgene-specific RT-PCR expression analysis revealing expression in bone and weak expression in the eye. (D) Northern Blotting comparing Krm2 expression in transgenic mice compared to wildtype littermates.


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