Figure_4.tif (110.19 kB)
Download file

Generation of BALB/cByJ-CD11B-Kif1cD2 transgenic (Tg-Kif1cD2) mice.

Download (0 kB)
posted on 27.12.2012, 02:00 by Roxana del Rio, Ryan D. McAllister, Nathan D. Meeker, Emma H. Wall, Jeffrey P. Bond, Vasileios C. Kyttaris, George C. Tsokos, Kenneth S. K. Tung, Cory Teuscher

(A) Schematic representation of the Kif1c gene used to generate the transgenic mice showing the promoter (CD11B/ITGAM), and the Kif1c gene, followed by the hGH/polyA signal sequence. Arrows indicate PCR-primers for screening. (B and C) Percentage of splenic F4/80+ (B) and CD11b+ (C) cells of Tg-Kif1cD2 and NLC. The analysis was performed on gated live cells according to their FSC vs. SSC profile. Statistical significance was determined using the Mann-Whitney U test. Data represent the mean ± SEM of at least 5 individual mice. (D) Kif1c expression in thioglycolate-induced adherent cells by Western blotting using whole-cell extracts and the anti-Kif1c mAb. Actin was used as a loading marker. (E) mRNA expression of Kif1c was measured from sorted TCRβCD19CD11b+ myeloid cells of CByJ mice and compared with TCRβCD19CD11b+ myeloid cells of D2 mice. β2-microglobulin and GAPDH were used as an endogenous control. Data represent the mean ± SEM of two experiments (pool of 5 animals/each).


Usage metrics