Gel electrophoresis of membrane-associated spore proteins.
Membrane preparations were obtained from dormant (D) and germinated (G) spores produced from three independent spore preparations (1, 2, and 3). Protein concentrations in membrane preparations were determined by quantitative amino acid analyses, and identical protein amounts were loaded onto a 9% polyacrylamide gel. Sizes of protein standard markers (M) are indicated on the left. Proteins were stained using Coomassie blue.