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Gas6 treatment increased TGF-β1 secretion, and this secretion induced Axl expression.

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posted on 19.02.2013, 19:23 authored by Young Sook Kim, Dong Ho Jung, Eunjin Sohn, Junghyun Kim, Jin Sook Kim

(a) Cells were treated with Gas6 for 24 h, and cell supernatants were collected for TGF-β1 ELISA analysis. All data are expressed as the mean ± S.E.M. (n = 4). ***P<0.001 vs. N-LDL (100 µg/ml). *P<0.05 vs. GO-LDL. (b) Cells were treated for 24 h and analysed by Western blotting using a Axl-specific antibody. Gas6-induced TGF-β1 expression (c) and secretion (d) were inhibited by PKC inhibitors. Cells were pretreated with PKC inhibitors for 30 min and treated with Gas6 for 24 h.