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GRFT and SVN inhibit HCVpp entry for different genotypes.

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posted on 21.05.2013, 01:30 by Yutaka Takebe, Carrie J. Saucedo, Garry Lund, Rie Uenishi, Saiki Hase, Takayo Tsuchiura, Norman Kneteman, Koreen Ramessar, D. Lorne J. Tyrrell, Masayuki Shirakura, Takaji Wakita, James B. McMahon, Barry R. O'Keefe

Huh7.5.1 cells were incubated for 3 hr with either HCV pseudoparticles(pp) generated with envelope glycoproteins of genotype 2a (JFH-1 and J6 strains) and genotype 1b (TH strain) or VSV-G pp in the presence of increasing amount of GRFT (A) or SVN (B). The inoculum was then removed, and the cells were further incubated. At 2 days post-inoculation, cells were lysed and processed to measure the luciferase activity. The results are presented as percentage of the infectivity relative to infectivity of HCVpp in the absence of carbohydrate binding proteins. Pseudotyped particles produced in the absence of envelope proteins were used as controls, representing <2% of the activity measured for HCVpp. The results are expressed as the mean ± SD in triplicate experiments.