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GM-CSF and IFN-γ levels of PBMCs from patients before surgery.

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posted on 06.09.2013, 01:50 by Savvas Petanidis, Doxakis Anestakis, Maria Argyraki, Margarita Hadzopoulou-Cladaras, Athanasios Salifoglou

In (A) GM-CSF ELISPOT of PBMCs was obtained from patients before surgery. Mononuclear cells were stimulated and were investigated for GM-CSF secretion. All experiments were carried out in triplicate. Results are presented as mean ± SD of the different groups. (B) IFN-γ ELISPOT of PBMCs was obtained from patients before surgery. Mononuclear cells were stimulated and were investigated for IFN-γ secretion. All experiments were carried out in triplicates. Results are presented as mean ± SD of the different groups. Large bar indicates comparison between patient stage D and stage D+Manumycin treatment samples.

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