GCV sensitivity to TK transfected cells.
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Cell viability assay by MTT was evaluated by transfecting the EGP2-TK and CMV-TK plasmids into the cell lines. Nthy-ori-3-1 (A), MIO-M1 (B), Y79 (C), WERI-Rb1 (D). After 48 hrs of transfection, followed by GCV treatment at different concentrations (1, 10, 50, 100 µM) for 24 hrs. The results were normalized against un-transfected cells and were expressed in percentage of cell viability. Cell viability was shown as percentage mean ± S.D (n = 3).