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GCV sensitivity to TK transfected cells.

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posted on 31.12.2013 by Ravikanth Danda, Gopinath Krishnan, Kalaivani Ganapathy, Uma Maheswari Krishnan, Khetan Vikas, Sailaja Elchuri, Nivedita Chatterjee, Subramanian Krishnakumar

Cell viability assay by MTT was evaluated by transfecting the EGP2-TK and CMV-TK plasmids into the cell lines. Nthy-ori-3-1 (A), MIO-M1 (B), Y79 (C), WERI-Rb1 (D). After 48 hrs of transfection, followed by GCV treatment at different concentrations (1, 10, 50, 100 µM) for 24 hrs. The results were normalized against un-transfected cells and were expressed in percentage of cell viability. Cell viability was shown as percentage mean ± S.D (n = 3).