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Fv1 incompatibility induces significant cell-mediated and humoral immune responses.

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posted on 23.03.2013, 00:32 by Kalani Halemano, Bradley S. Barrett, Sam X. Li, Michael S. Harper, Diana S. Smith, Karl J. Heilman, Mario L. Santiago

(A–D) Apobec3-deficient 129P2 mice were infected with 7500 SFFU B-tropic FV (FV-B) or DMEM (mock). Mouse splenocytes were analyzed by flow cytometry at 28 dpi. (A–C) Cell mediated immune responses. Splenocytes were stimulated with PMA/ionomycin for 5 h prior to flow cytometric analysis. (A) Representative pseudo-color plots and histograms. Flow cytometry plots show the gating strategy for CD4+ and CD8+ lymphocytes. (B) Intracellular IFN-γ and (C) Surface CD107a in lymphocyte subsets. (D) Splenic germinal center (GL7+ IgD-) B cells. (E-F) Analysis of FV-specific antibody responses in 28 dpi plasma of 129P2 Apobec3 KO mice following infection with B-tropic FV (7500 SFFU) or NB-tropic FV (500 SFFU). (E) FV-specific IgG endpoint titers as measured by ELISA. (F) NAb titers. For panels B to F, the black bars represent the mean, and each dot corresponds to an individual mouse. Data were analyzed using a 2-tailed Student’s t test with p<0.05 considered as significant. Data are representative of two independent experiments.


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