Figure_13.tif (388.73 kB)
Functional activity of MtbC406S mutated protein.
figure
posted on 2010-11-30, 02:10 authored by Kajal Gupta, Prasun Kumar, Dipankar Chatterji(A) LC-ESI-MS of DTT reduced MtbC406S digested with trypsin and chymotrypsin. The m/z 760.9 shows the substitution of Cys406 to Serine406. (B) (a) MtbC406S protein: MALDI TOF analysis, in the positive ion detection mode. No peaks are obtained at 691 (M+H)+ and 713 (M+Na)+ for c-di-GMP, 709 (M+H)+ and 731 (M + Na)+ for pGpG (b) MtbDGC protein for control: MALDI TOF analysis, in the positive ion detection mode, ions were detected at 691 (M+H)+ and 713 (M+Na)+ for c-di-GMP (c) 709 (M+H)+ and 731 (M + Na)+ for pGpG.
