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Functional SMEZ is required to stimulate production of cytokines from human tonsil cells.

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posted on 01.10.2012, 01:52 by Claire E. Turner, Mary Sommerlad, Karen McGregor, Frances J. Davies, Bruno Pichon, Deborah L. W. Chong, Leili Farzaneh, Matthew T. G. Holden, Brian G. Spratt, Androulla Efstratiou, Shiranee Sriskandan

Human tonsil cell suspensions were cultured with bacterial cell-free culture supernatants from GAS-M3control(white bars), GAS-M3smeZ-M89 (black bars) and GAS-M3smeZ-M3 (gray bars). After 2 and 5 days incubation cell-free media were obtained from cultures and production of TNFα, TNFβ, IL-10, IL-17 and IFNγ were measured by ELISA. Horizontal dotted lines represent the mean level of cytokines produced after co-culture with bacterial cell-free culture supernatants from GAS-M1control on day 2 and day 5. Mean (+ standard deviation) of three replicates measured in duplicate. Representative of two experiments performed on different donors. Statistical analysis was performed using ANOVA with Bonferroni multiple comparison.

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