Functional SMEZ is required to stimulate production of cytokines from human tonsil cells.
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Human tonsil cell suspensions were cultured with bacterial cell-free culture supernatants from GAS-M3control(white bars), GAS-M3smeZ-M89 (black bars) and GAS-M3smeZ-M3 (gray bars). After 2 and 5 days incubation cell-free media were obtained from cultures and production of TNFα, TNFβ, IL-10, IL-17 and IFNγ were measured by ELISA. Horizontal dotted lines represent the mean level of cytokines produced after co-culture with bacterial cell-free culture supernatants from GAS-M1control on day 2 and day 5. Mean (+ standard deviation) of three replicates measured in duplicate. Representative of two experiments performed on different donors. Statistical analysis was performed using ANOVA with Bonferroni multiple comparison.