Foxp1 knockdown does not affect neuronal differentiation.

E14.5 mouse embryos were electroporated with Foxp1 shRNA-b or shRNA-Scr, and brains were fixed at P2. Coronal sections were immunostained for Satb2 (A) and Cux1 (B). a, b, c, High-magnification images of the boxed regions in the left panels. a, b, c in (A) and (B) represent layer II-IV, layer V and white matter, respectively. (C), (D), Immunofluorescence staining of Ctip2 and Tbr1 in brain sections from control and Foxp1 shRNA-b. (C)-a, (D)-a, High-magnification images of the boxed regions in the left panels. Scale bar, 50μm in (A), (B), (C) and (D).