FldP mitigates H₂O₂-induced cell death in a mutT-deficient P. aeruginosa.

The mutT mutant strain (transformed with the empty plasmid p2) was tested for resistance to H₂O₂, and compared with its wt parental strain (harboring p2), and with mutT mutants transformed with p2-fldP or p2-isiB. Bacterial cultures were normalized to ∼10⁸ cells and then incubated with 50 mM H₂O₂ for 30 min at 37°C. Viability was estimated as described in Materials and Methods. Measurements were carried out in triplicate for two independent experiments, and the results were expressed as means with their ± SEM. Statistically significant differences (P<0.05) are identified by * (one-tailed Mann-Whitney test).