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Flagellar expression and motility of P. syringae pv. averrhoi strains.

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posted on 23.07.2012, 01:14 authored by Chia-Fong Wei, Shih-Tien Hsu, Wen-Ling Deng, Yu-Der Wen, Hsiou-Chen Huang

(A) TEM images of P. syringae pv. averrhoi strains HL1 (a), PA5 (b), PA5ΔfliC (c) grown for 24 h on KB plates, deposited on TEM grids. Bars represent 200 nm and the arrow indicates the flagella only presented in wild type PA5. (B) Swimming assay. Bacterial strains were incubated for 2 days at 23°C on soft agar of hrp minimal medium or LB containing 10 mM MgCl2 plate (as indicated as LB). (C) Immunoblot analysis of flagellin expression in HL1, PA5, fliC mutant and complemented strain was detected by anti-FliC antiserum. Except for the flagellin of HL1 induced in hrp minimal medium (MM), the others were prepared from cultures in KB medium. pNCHU1039: pBBR1MCS5 carrying fliC.