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Figure shows the general process of probe ligation followed by FLP size-exclusion purification.

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posted on 2013-02-20, 06:43 authored by Michael A. Jensen, Lauren Jauregui, Ronald W. Davis

To make each probe, the variable, bridge and common strands are first combined in the presence of a ligase mix (e.g. Fast-Link or Quick Ligase); the reaction is then carried out at RT for 10 min. Afterward, the sample is passed through P-10 polymeric resin where unincorporated species are thus retained, and FLP eluted. The final purified, desalted product of pooled ODN probes is now ready for downstream application.

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