Fibroblast specific antibody staining.
Panel A showed the representative images of fibroblast antibody (Fibroblast Surface Protein in red-APC) staining on human CSCs before (a) and after (b) c-kit MACS. The 2nd antibody-APC (c, 2nd AB only) and human fibroblast cell line (d) were used as positive and negative controls, respectively. Cell nuclei were stained in blue (DAPI). Scale bars = 20 µm. Panel B showed the Mean±SEM data of ICC staining from three patients (n = 3 patients). ***: p<0.001 compared with control (Before c-kit MACS).