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Fibroblast specific antibody staining.

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posted on 20.02.2013, 11:23 by Jia-Qiang He, Duc Minh Vu, Greg Hunt, Atul Chugh, Aruni Bhatnagar, Roberto Bolli

Panel A showed the representative images of fibroblast antibody (Fibroblast Surface Protein in red-APC) staining on human CSCs before (a) and after (b) c-kit MACS. The 2nd antibody-APC (c, 2nd AB only) and human fibroblast cell line (d) were used as positive and negative controls, respectively. Cell nuclei were stained in blue (DAPI). Scale bars = 20 µm. Panel B showed the Mean±SEM data of ICC staining from three patients (n = 3 patients). ***: p<0.001 compared with control (Before c-kit MACS).

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