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Fatty and mycolic acid profiles in D5 MUT.

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posted on 2014-06-20, 03:13 authored by Bernardo Bazet Lyonnet, Lautaro Diacovich, Matías Cabruja, Fabienne Bardou, Annaïk Quémard, Gabriela Gago, Hugo Gramajo

A) de novo fatty acid and mycolic acid biosyntheses. At the indicated times, aliquots from D5 MUT cultures incubated in absence or presence of ATc were labelled with [14C]-acetate for 1 hour at 37°C. Fatty acid and mycolic acids methyl esters were extracted from samples containing equivalent amounts of bacteria and were analyzed by TLC. B) Quantification of the radiolabelling intensity of the TLC showed in panel A.