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Fatty acid supplementation for wild type and SREB∆.

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posted on 26.06.2015, 04:48 authored by Amber J. Marty, Aimee T. Broman, Robert Zarnowski, Teigan G. Dwyer, Laura M. Bond, Anissa Lounes-Hadj Sahraoui, Joël Fontaine, James M. Ntambi, Sündüz Keleş, Christina Kendziorski, Gregory M. Gauthier

(A) Percentage of WT and SREB∆ with yeast morphology, germ tube development, and hyphae at 22°C for cells grown in media supplemented with 0.5 mM palmitic acid (16:0) or 0.5 mM stearic acid (18:0). Controls included DMSO only and cells grown in media without DMSO or saturated fatty acid (untreated). At least 200 cells were counted in duplicate. Results were averaged from 2 independent experiments. (B) Percentage of filaments that contained 0–4 or 5 LDs per 10 μm segment at 24 and 48-hrs 22°C for WT and SREB∆ cells (untreated, DMSO only, 16:0 and 18:0). LDs were quantified per 10 μm segment along the length of filaments from at least 30 cells. Results were averaged from 2 independent experiments. (C) BODIPY 493/503 staining of lipid droplets at 24 and 48-hrs 22°C for WT and SREB∆ cells (untreated, DMSO only, 16:0 and 18:0). Scale bar equals 10 μm.

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