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FRAP analysis reveals incorporation of Httex1-GFP mutants into microcomplexes.

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posted on 28.12.2010, 00:06 by Patrick Lajoie, Erik Lee Snapp

(A) Fluorescent images of N2a cells transiently transfected 16 h (to minimize the number of IBs) with Httex1-GFP constructs containing 23, 73 or 145 polyQ repeats arte shown before (prebleach), immediately after (t = 0) and after recover (t = 3.8 s). (B) D values (µm2/s) of single N2a cells transiently transfected with Httex1-GFP constructs containing 23, 73 or 145 polyQ repeats and analyzed by FRAP. (C) Plot of D values (µm2/s) and camera gain settings for N2a cells transiently expressing Q23, Q73 or Q145 Httex1-GFP. (D) D values (µm2/s) of single N2a cells transiently cotransfected with Httex1-GFP constructs containing 23, 73 or 145 polyQ repeats and cytosolic mCherry and analyzed by FRAP. (E) D values (µm2/s) for N2a cells transiently expressing Q23, 73 or 145 Httex1-GFP for 16, 48 and 72 h. Bar = 20 µm.

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