FLO11 is required for rough morphology and shows differential expression across genetic backgrounds.
(A) Deletion of FLO11 leads to smooth morphology in both the five- and six-way genetic interaction backgrounds. (B) RT-PCR of FLO11 and the housekeeping gene ACT1 in multiple genetic backgrounds. FLO11 is not expressed in BY or 3S, but is expressed in recombinants that carry the five- and six-way genetic interactions. FLO11 is also expressed in 3S ira2Δ2933 and 3S sfl1Δ strains.