FKBP51 enhances NDV-dependent expression of type I IFN.
(A) Knockdown of FKBP51 in MEF cells. Total cell lysates from FKBP51-knockdown cells (siFKBP51) and control knockdown cells (siControl) infected with NDV or without infection were analyzed by western blotting using anti-FKBP51 antibody (upper panel) and anti-tubulin antibody (lower panel) as the loading control. One representative experiment of three is shown. (B) FKBP51-knockdown and control MEF cells were infected with NDV (MOI = 10). At 8 h after infection, the expression of IFNβ was evaluated by qPCR and normalized to the expression of β-actin mRNA. Data are the means ± SD of triplicate determinations and are representative of three independent experiments. *P<0.05; Student’s t test with a two-tailed distribution and two-sample equivalent variance parameters.