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FFUJ_09118 is a functional homologue of S. cerevisiae Gap1.

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posted on 24.04.2015 by Andreas Pfannmüller, Dominik Wagner, Christian Sieber, Birgit Schönig, Mélanie Boeckstaens, Anna Maria Marini, Bettina Tudzynski

Nitrogen utilization growth assay of S. cerevisiae mutants gap1Δdip5Δ and gap1Δssy1Δ complemented with putative GAP-encoding genes of F. fujikuroi (FFUJ_01137, FFUJ_05331, FFUJ_09118, FFUJ_11370) and PcGAP1 of P. chrysogenum. Fresh cells of tested yeast strains were adjusted to an optical density of 1 and (A) plated on yeast minimal agar with 1 mM citrullin (Cit), 10 mM aspartate (Asp), 10 mM phenylalanine (Phe), 1 mM tyrosin (Tyr) or 10 mM isoleucine (Ile) or (B) a series of 10-fold dilutions (left to right) were dropped on yeast minimal agar with 1 mM ammonium sulfate ((NH4)SO4) or glutamate as sole nitrogen source. Plates were incubated for 4 days at 30°C.

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