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F2 mice display different rates of TB progression.

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posted on 04.05.2010, 00:26 by Irina V. Lyadova, Evgeny N. Tsiganov, Marina A. Kapina, Galena S. Shepelkova, Vasily V. Sosunov, Tatiana V. Radaeva, Konstantin B. Majorov, Natalya S. Shmitova, Henk-Jan van den Ham, Vitaly V. Ganusov, Rob J. De Boer, Rachael Racine, Gary M. Winslow

F2 mice were challenged i.t. with 103 CFU of Mtb. Weight was monitored once a week. A, Kinetics of weight change. 100% - weight on day 1 post-infection. Shown are representative results obtained in two (n = 52) independent experiments that included mice of both sexes. B–D, Mycobacterial load (B), lung pathology (C), and lung cell viability (D) in mice displaying different degree of wasting. Severely wasting mice (wasting by more than 20%) are indicated by blue circles. Lines show the predictions of the linear regression when all mice are included in the analysis (dashed blue lines) or when severely wasting mice are excluded from the analysis (solid black lines). ρ, Spearman correlation coefficient; p, p-value for ANOVA. E, F, Examples of lung tissue sections and lung cell flow cytometry representing two extremes with mild and severe pathology. Numbers in F indicate the percentages of dead (left) and live (right) cells. G, H, Long-term monitoring of F2 mice (n = 30, two independent experiments). G, The kinetics of weight change. H, Comparison of mycobacterial loads observed on days 24 and 140 post-infection.


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