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Expression profiles of GABAergic signaling molecules.

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posted on 20.02.2013, 17:54 by Yukari Nakamura, Eiichi Hinoi, Takeshi Takarada, Yoshifumi Takahata, Tomomi Yamamoto, Hiroyuki Fujita, Saya Takada, Syota Hashizume, Yukio Yoneda

(A) Total RNA was extracted from 3T3-L1 cells cultured for 2 to 8 days and epididymal WAT of male mice, followed by RT-PCR using specific primers for each molecule. (B) Both 3T3-L1 cells and EF were cultured until confluence, followed by detection of GABABR1 subunit protein on immunoblotting analysis. Mouse whole brain was used as a positive control. Expression of mRNA and corresponding protein for GABABR1 subunit was constitutively seen in adipocytic cells.


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