Expression patterns of GsPPCK3 in Glycine soja.

a. Expression levels of GsPPCK3 were up-regulated by alkali stress in both roots and leaves. Total RNA was extracted from leaves and roots of the 3-week-old Glycine soja seedlings treated with 50 mM NaHCO₃ (pH 8.5) for the indicated time points, respectively. Relative transcript levels were determined by quantitative real-time PCR analysis with GAPDH as an internal control. The mean values from three fully independent biological repeats and three technical repeats were shown. *P<0.05; **P<0.01 by Student’s t-test. b. Tissue expression specificity of GsPPCK3 in Glycine soja. Total RNA was extracted from different tissues of Glycine soja seedlings. Significant differences (P<0.05 by Duncan’s Multiple Range Test) were indicated by different lowercase letters.