Expression of collagen-I and integrins in the fetal kidney.

(A) Immunostaining of E14.5 kidney with anti-TROP2 (green) and anti-collagen-I (red) Abs and nuclei (blue). Higher magnification images of the boxed regions are shown underneath. Arrowheads indicate collagen-I surrounding the ureteric trunks. Scale bars = 100 µm. (B) Quantitative RT-PCR analysis of integrin subunits in freshly isolated EpCAM⁺TROP2^low(+) and EpCAM⁺TROP2^high(++) cells from E14.5 kidney. The relative gene expression of integrin/GAPDH is shown. Error bars are s.d.