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Expression of VH3, D, and J family genes.

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posted on 2014-01-07, 03:23 authored by Elisabeth Bowers, Ronald W. Scamurra, Anil Asrani, Lydie Beniguel, Samantha MaWhinney, Kathryne M. Keays, Joseph R. Thurn, Edward N. Janoff

Data are shown as group medians among HIV-1-seronegative control subjects (n = 10; black bars or circles), aviremic patients (n = 6; gray bars or squares), and viremic HIV-1-infected patients with detectable plasma HIV-1 RNA (n = 15; white bars or triangles). Values were calculated from individual mean percent expression for each gene. A) VH3 family gene expression. “Other” includes VH3-13, -20, -43, -49, -64, -65, -66, -72, and unidentified genes which ranged from 0.4% to 12.6% expression. P values were calculated by Mann-Whitney test. Significant p values were found for VH3-7 (p = 0.05), VH3-9 (p = 0.05), VH3-23 (p = 0.03), and VH3-53 (p = 0.007). B) VH3-23 expression. Each point represents the mean percentage of VH3-23/total VH3 genes for each patient. The solid bar indicates the group median. C) D gene expression was calculated as with VH3 gene expression. “Other” includes D1-1, D1-7, D1-14, D1-20, D2-2, D2-8, D2-21, D3, D3-9, D3-16, D4, D4-b, D4-11, D4-23, D5-12, D6-6, and D6-25 which ranged from 0.4% to 4.3% expression. “No ID” (12.5–18.0% of sequences) indicates that gene identification could not be assigned. P values were calculated by Mann-Whitney test. Significant overall p values were found for D3-22 (p = 0.04), D5-24 (p = 0.05), and D6-19 (p = 0.04). D) J gene percent expression was calculated as described above. “Other” includes JH1, JH4a, JH4d, JH6a, and JH6c which ranged from 0.2% to 6.7% expression. Statistics were also calculated as above. A significant overall p value was found for JH6b (p = 0.01).