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Expression of PDGFRα enhances entry of HCMV TR into epithelial and endothelial cells.

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posted on 19.02.2013 by Adam L. Vanarsdall, Todd W. Wisner, Hetian Lei, Andrius Kazlauskas, David C. Johnson

A) Human epithelial cells (ARPE-19), B) human endothelial cells (HUVECs), C) human foreskin fibroblasts (HFF), D) owl monkey kidney cells (OMK), and E) rat retinal epithelial cells (Rat-RPE) were transduced with Ad vectors expressing tet-trans, PDGFRα, or EGFR using 50 PFU/cell for each Ad vector for 24 hr. The cells were infected with HCMV TR using 10 IU/cell and 1 IU/cell for HFF cells then fixed, permeabilized, and stained for HCMV IE-86 immediate early protein. The percent infected cells was calculated by counting the number of IE-86+ cells in three fields from three separate wells involving two separate experiments, comparing to the total number of cells in each field then averaging the numbers. Standard deviations are indicated.

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