Expression of PDGFRα enhances entry of HCMV TR into epithelial and endothelial cells.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
A) Human epithelial cells (ARPE-19), B) human endothelial cells (HUVECs), C) human foreskin fibroblasts (HFF), D) owl monkey kidney cells (OMK), and E) rat retinal epithelial cells (Rat-RPE) were transduced with Ad vectors expressing tet-trans, PDGFRα, or EGFR using 50 PFU/cell for each Ad vector for 24 hr. The cells were infected with HCMV TR using 10 IU/cell and 1 IU/cell for HFF cells then fixed, permeabilized, and stained for HCMV IE-86 immediate early protein. The percent infected cells was calculated by counting the number of IE-86+ cells in three fields from three separate wells involving two separate experiments, comparing to the total number of cells in each field then averaging the numbers. Standard deviations are indicated.