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Expression of PDE6β1-314fs*50 in transgenic X. laevis rods photoreceptors.

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posted on 23.04.2014, 15:35 by Gaël Manes, Pallavi Cheguru, Anurima Majumder, Béatrice Bocquet, Audrey Sénéchal, Nikolai O. Artemyev, Christian P. Hamel, Philippe Brabet

A. left - EGFP fluorescence in living photoreceptor cells expressing PDE6β1-314fs*50, center - DIC image, right - EGFP-fluorescence/DIC overlay. Bar - 10 µm. B. Co-localization of PDE6β1-314fs*50 (green) with the rod outer segment marker Wheat Germ Agglutinin (red) in cryosections of transgenic retina. Cryosections were counterstained with TO-PRO3 nuclear stain (blue). Bar - 10 µm. C. Isotonic (I), hypotonic (H) extracts of transgenic PDE6β1-314fs*50 retinas were analyzed by immunoblotting with anti-GFP B-2 monoclonal antibody.