Figure_4.tif (2.2 MB)

Expression of GIT1-C affects cell morphology and the distribution of endogenous liprin-α1.

Download (0 kB)
figure
posted on 13.06.2011 by Claudia Asperti, Veronica Astro, Emanuela Pettinato, Simona Paris, Angela Bachi, Ivan de Curtis

(A) COS7 cells transfected for one day with either FLAG-GIT1, FLAG-GIT1-C, or FLAG-βGalactosidase were re-plated for 1 h on FN. Immunofluorescence for the transfected proteins (FLAG), paxillin, and phalloidin staining for F-actin. Scale bar, 20 µm. Below, 3-fold enlargements of areas from cells stained for paxillin (arrowheads in the corresponding cells above) are shown. (B) Expression of GIT1-C induces a significant increase of cell spreading on FN. Bars are means ± SEM (n = 116–121 cells per condition); *P<0.05. (C) Cells transfected with either FLAG-βGalactosidase or FLAG-GIT1-C were used for triple immunofluorescence staining with antibodies for endogenous liprin-α1, paxillin, and transfected proteins (FLAG): endogenous liprin-α1 accumulates at the edge of GIT1-C-transfected cells. Scale bar, 5 µm.

History

Licence

Exports