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Expression and purification of four OBPs and two CSPs of

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posted on 2013-12-23, 03:57 authored by Bing Wang, Li Guan, Tao Zhong, Kebin Li, Jiao Yin, Yazhong Cao

H. oblita. SDS-PAGE electrophoretic (15% separation gel) (A) and western blotting (B) analysis of expressed recombine proteins. M: Molecular weight marker of 100, 70, 50, 40, 30, 25, 14 kDa; 1-2: before and after induction of the bacterial culture with IPTG; 3: supernatant; 4: inclusion bodies; 5: Purified fusion protein; 6: Purified protein cleaved His-tag by rEK.