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Expression analysis of B. cockerelli aquaporin candidates in guts.

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posted on 29.10.2014 by Freddy Ibanez, Joseph Hancock, Cecilia Tamborindeguy

A: In situ hybridization of candidate mRNAs with digoxigenin-labelled antisense and sense RNA probes (original magnification: ×5, bars = 200 µm). B: Relative gene expression levels of B. cockerelli aquaporin candidate genes normalized to the expression value of the RPS18 gene. Data points represent means ± SD of two independent experiments performed in duplicates. C. Light micrograph of B. cockerelli alimentary canal showing characteristic structures. FC: filter chamber, M: midgut, MA: midgut appendages.