Experimental protocols of thioacetamide (TAA)-induced hepatic fibrosis in rats pretreated with Sho-Saiko-To (SST).
Eight-week-old male rats were intraperitoneally injected with TAA (200 mg/kg) twice weekly at weeks 1 and 2 and then once at weeks 5 and 6. A cohort of rats receiving vehicle without treatment of SST (TAA only) was assigned as Group 1. Group 2 (TAA +0.25 g/kg SST) was administered moderate dose (0.25 g/kg) of SST two days before the first TAA exposure and sustained for 6 weeks. Group 3 (TAA +1 g/kg SST) was administered high dose (1 g/kg) of SST.