Experimental design of the study.
(A) CHI mice were impacted in the right hemisphere (red), followed by an identical impact in the left hemisphere in rmCHI mice. (B) A representative image of the brain and injury site (red bar) after impact in our CHI model. No overt tissue damage is observed after injury. (C) Mice underwent neurological and behavioral testing in the acute phase (1–7 dpi) and also at a chronic phase (90 dpi). Testing in the acute phase consisted of the open field (OF), balance beam (BB), foot fault (FF), and rotarod (RR). Testing at 90 dpi consisted of the same acute tasks above, as well as the elevated zero maze (ZM), water maze (WM), tail suspension test (TST), forced swim test (FST), social recognition test, and scent-marking test. At the end of behavioral testing, animals underwent perfusion fixative for high resolution MRI.