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Experimental design.

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posted on 2009-11-10, 01:56 authored by Alan Gillett, Klio Maratou, Chris Fewings, Robert A. Harris, Maja Jagodic, Tim Aitman, Tomas Olsson

DA (n = 4) and PVG (n = 4) female rats were immunized with MOG (day 0) to induce EAE. At day 7, before clinical symptoms, lymph nodes were extracted and either directly harvested (ex vivo) or stimulated with autoantigen (MOG re-stimulated). cRNA was prepared and gene expression tested using 16 Affymetrix Rat Exon 1.0 ST Arrays.

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