Experimental UTI with CONS292 recapitulates the spectrum of pathology observed in spontaneously infected Mgb-/- mice.
(A) WT bladder exhibits regenerative apical urothelium and focal mononuclear submucosal infiltrate 14 dpi with CONS292. H&E, 20x objective (B)Mgb-/- bladder exhibits epithelial thickening, and desquamation with luminal crystals and submucosal edema 14 dpi with CONS292. H&E, 20x objective (C) Focal inflammatory cellular infiltrate in the urothelium and perihilar fat (F) was the only evidence of pathological changes in a WT kidney 14 dpi with CONS292. (P, renal papilla). Green dotted line (inset) demarcates the urothelial basement membrane. H&E, 5x objective; inset 20x objective (D)Mgb-/- kidney 14 dpi with CONS292 exhibits widespread changes including areas of dense mixed cellular infiltrate in the interstitium and tubules (inset1) as well as urothelial thickening, suburothelial inflammatory cells, and fibrosis (inset2). Nests of inflammatory cells are found scattered throughout the parenchyma (yellow ovals). Urothelial vacuolization is also present (orange circle, inset2). H&E, 5x objective; inset1 40x objective; inset2 20x objective.