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Expansion of Th17 but not and Th1 cells in the draining lymph nodes of col II-immunized CD38 KO mice.

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posted on 20.02.2013, 07:39 by Jorge Postigo, Marcos Iglesias, Daniela Cerezo-Wallis, Antonio Rosal-Vela, Sonia García-Rodríguez, Mercedes Zubiaur, Jaime Sancho, Ramón Merino, Jesús Merino

WT and CD38 KO mice male mice were immunized with col II-CFA. For intracellular cytokine staining, lymphocytes from paraaortic lymph nodes were stimulated 3 weeks after immunization with phorbol myristate acetate and ionomycin in the presence of GolgiStop solution. (A) Representative histograms of CD4+IFNγ+ Th1 and CD4+IL-17+ Th17 cells, determined by flow cytometry, in the different experimental groups. The percentage of each population is indicated. (B) Number of CD4+IFNγ+ Th1 (left panel) and CD4+IL-17+ Th17 (right panel) cells in the draining lymph nodes of non-immunized (NI; •) and 3 weeks post-immunized (○) mice. Values of individual mice are expressed. Bars represent the mean value of each examination. Statistic differences are indicated as follow: ns: non-significant, *p<0.05, **p<0.01, *** p<0.001.