Example of movie images of males courting females, including corresponding errors in automatic classifications by body orientation and identity.
(A) Examples of movie images with body orientations annotated (triangle apex denotes the position of the fly's head) that were extracted with the CADABRA software system. (B) Error rates of body orientations calculated for individual flies from movies recorded for males courting females within the chambers with sloped (red) and vertical (black) walls. Error rates from the chambers with vertical walls were decomposed into erroneous flips in orientation during periods when individual flies were either on the floor or on the wall. Medians (blue) and 25 and 75 percentiles are shown (black box). (C) Examples of movie images with identity annotated while males (blue) courted females (red). Trajectories represent the location of flies for the past 30 frames ( 1 s). Swaps in identity are denoted by the discontinuities in the trajectories and changes in color between triangles representing past locations of flies, and therefore can be compared to a movie image that has been corrected (left). (D) Error rates for the classification of identity between pairs of male and females from movies recorded within the chambers with sloped (red) and vertical (black) walls. Error rates from the chambers with vertical walls were decomposed into swaps in identity during periods when both flies were on the floor, both on the wall, and split with one fly on the floor and the second on the wall. Medians (blue) and 25 and 75 percentiles are shown (black open box). The rate of errors were similar between Wall vs. Split, Mann-Whitney U, p = 0.628; Wall vs. Vertical, Mann-Whitney U, p = 0.864; and Split vs. Vertical, Mann-Whitney U, p = 0.521.