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Examination of the interaction of Rre3, CopR, NrsR and CcaR with Trx in E. coli cells.

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posted on 2015-03-16, 05:15 authored by Taro Kadowaki, Yoshitaka Nishiyama, Toru Hisabori, Yukako Hihara

Soluble proteins from the E. coli Origami2 strain (Control), the strain expressing only TrxMC35S (Trx), the strain expressing only a TF and the strain expressing both TrxMC35S and a TF were separated by non-reducing 12% SDS-PAGE and immunoblot analysis was performed. (A) Rre3, (B) CopR, (C) NrsR and (D) CcaR were detected using a His-tag antibody (left panel) and Trx was detected with S-protein (right panel). ± indicates with or without 100 mM DTT treatment. Black and white arrows indicate the TF monomer and the Trx monomer, respectively.

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