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Evaluation of busulfan pre-conditioning in mice.

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posted on 17.09.2013, 01:31 by Jean Chevaleyre, Pascale Duchez, Laura Rodriguez, Marija Vlaski, Arnaud Villacreces, Véronique Conrad-Lapostolle, Vincent Praloran, Zoran Ivanovic, Philippe Brunet de la Grange

Mice received increasing doses of busulfan (2 injections) and were weighted prior and after 24 hours following each injection. A. Percentage (%) of weight relative to weight prior injection (---) and measured 24 hours after the first (▪) and the second (□) injection of different doses of busulfan. B. Percentage of weight loss according to gender 24 hours after the first (▪) and the second (□) injection. C. Survival of mice according to gender. D-E. Mice received 1000 non manipulated CD34+ cells by i.v. caudal injection and were analyzed 8 weeks later for huCD45 chimerism (D) and huCFU content (E) in their bone marrow. Each mouse represented by (●), Median represented by (─).

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