Est2p telomere binding is eliminated in mutants lacking both the G1 and the late S/G2 phase recruitment pathways.

Methods are the same as in Figure 1 legend. A. Est2p binding to VII-L telomere in synchronous tlc1Δ48 cdc13-2 (black triangles) versus WT (white squares), or untagged (white triangles) cells. Est2p telomere binding in the double mutant was not different from the no-tag control (P = 0.11 to 0.85) except at the 0 min time point (P = 0.039). B. Est2p binding to VII-L (top) or VI-R (bottom) telomeres in asynchronous WT and mutant cells. Bar graphs show average Est2p association with telomere VII-L (dark grey) or VI-R (light grey). Abbreviations are Δ48, tlc1Δ48; 13-2, cdc13-2; 135, yku80-135i, SD, tlc1-SD; SC, tlc1-SC; BD, tlc1-BD. For double mutants, there is a slash between the two alleles as in Δ48/est1Δ which stands for tlc1Δ48 est1Δ. Error bars indicating ±one standard deviation from that average. Est2p binding was indistinguishable in tlc1Δ versus the double mutants yku80-135i tlc1-SD or yku80-135i tlc1-BD cells (P values ranged from 0.28 to 0.85) while Est2p binding in the double mutant yku80-135i tlc1-SC was significantly greater than in tlc1Δ (P = 0.003, VII-L; 0.0001, VI-R).