Eps8 is required for Wnt3a-mediated axonal remodeling.
(A) Eps8 promotes growth cone enlargement and increases F-actin accumulation in growth cones. Pseudocolor images show the different levels of F-actin in growth cones of Eps8-expressing and control GFP-expressing cells. Scale bar: 5μm. (B and C) Growth cone area is larger in Eps8-expressing cells than in controls cells, as is the percentage of growth cone area with high F-actin fluorescence. (D) DRG neurons expressing scramble or Eps8 shRNAs were treated with Wnt3a for 2 hrs. Scale bar: 5μm. (E) In scrambled shRNA-expressing cells Wnt3a increases the percentage of cells that show axonal remodelling (% of cells remodelled) and (F) promotes growth cone enlargement. In contrast, neurons expressing Eps8shRNAs do not respond to Wnt3a. *p<0.05, **p<0.01, ***p<0.001.