Enzyme activity assays of ScRIB7 mutants.
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A, Relative reductase activity of ScRIB7 mutants toward DAROPP. B&C, Relative reductive activity of T35K mutant and wild type ScRIB7 toward DAROPP or AROPP as substrates. DAROPP and AROPP molecular structures are shown in the inset. Results are presented as means ± S.D. of three independent experiments.