Fig_5.tif (495.96 kB)

Enhancement of chaperone activity of M. leprae HSP18 induced by ATP and its non-hydrolysable analog, ATP-γS.

Download (0 kB)
figure
posted on 26.03.2015 by Sandip Kumar Nandi, Ayon Chakraborty, Alok Kumar Panda, Sougata Sinha Ray, Rajiv Kumar Kar, Anirban Bhunia, Ashis Biswas

Thermal aggregation of 0.06 mg/ml CS at 43°C (panel A) and DTT-induced aggregation of 0.05 mg/ml lysozyme at 37°C (panel B) in the absence or presence of different HSP18 preparations. Trace 1:Client protein (CP) alone; Trace 2: CP + 1 mM ATP; Trace 3: CP + 1 mM ATP-γS; Trace 4: CP+ HSP18; Trace 5: CP + HSP18 preincubated with 1 mM ATP; Trace 6: CP + HSP18 preincubated with 1 mM ATP-γS. Each data point is the average of triplicate measurements. Panels C and D represents the percent protection ability of different HSP18 preparations against CS and lysozyme aggregation. The chaperone:client protein ratio was 1:1 (w/w) for CS and lysozyme aggregation assays. Data are the means ± standard deviation from triplicate measurements. **p< 0.005 and ***p<0.0005.

History

Licence

Exports