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Engineering of a TgCEL mouse model and the chronic cerulein protocol.

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posted on 03.04.2013, 04:16 authored by Helge Ræder, Mette Vesterhus, Abdelfattah El Ouaamari, Joao A. Paulo, Fiona E. McAllister, Chong Wee Liew, Jiang Hu, Dan Kawamori, Anders Molven, Steven P. Gygi, Pål R. Njølstad, C. Ronald Kahn, Rohit N. Kulkarni

(A) Structure of the construct that was linearized and subjected to pronuclear injection into fertilized C57BL/6 mice. (B) Pancreatic mRNA expression of human carboxyl-ester lipase, hCEL, normalized to beta actin in TgCEL mice compared to controls (left panel) and the relative expression of hCEL to murine Cel (mCEL) in TgCEL mice compared to controls (right panel). (C) X-gal staining (blue; upper panel) and immunofluorescent histochemical analysis (lower panel) of pancreas sections of bitransgenic LacZ+/−,elastase-Cre+/− mice confirmed induction by the elastase promoter of Cre expression in acinar tissue and in 10–15% of β cells in pancreatic islets from two different mice (Green, LacZ; red, insulin; blue, nuclei). (D) Chronic cerulein protocol; PBS, Phosphate buffered saline; WT wild type; IPGTT, intraperitoneal glucose tolerance test; BW, body weight.